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KMID : 0359319750150010075
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Korean Journal of Veterinary Research
1975 Volume.15 No. 1 p.75 ~ p.81
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Studies on Culture of Leptospires
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Abstract
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The rate of growth of Leptospires were studied by culturing in Korthof´s media and Cox´s liquid media which were treated at four different temperatures. The effect of thiamine hydrochleride in the culture of leptospires were also studied.
The results obtained were summerized as followings:
1. The best growth of Leptospira icterohaemorrhagiae and Leptospira canicola was observed in Kcrthof´s media with the rabbit serum heated for 60 minutes at 56℃.
2. A good degree of growth of Leptospira icterohaemorrhagiae and Leptospira caaicola was observed in both Cox´s liquid media with rabbit serum heated for 30 minutes at 100℃ and with fresh rabbit serum.
3. The growth of Leptospira was enhanced by adding small amount of thiamine to the both media which were shown to be unsatisfactory for the growth of the organisms.
4. Culture of leptospires were attained by simply heating for 30 minutes at 100℃ both Korthof´s media and Cox´s liquid media containing rabbit serum obtained without aspetic procedures.
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